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KMID : 0367419690100010037
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Journal of Korean Pediatric Society
1969 Volume.10 No. 1 p.37 ~ p.41
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Studies on the Measurement of Serum Bilirubin by the Eberlein´s Method and a Modified Method
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鄭性煥/Chung, Sung Whan
趙永琪/金昌世/Cho, Young Kee/Kim, Chang Se
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Abstract
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Eberlein´s simple partition method is in general use for the measurement of free bilirubin, bilirubin mono- and diglucuronide which is used for the preparation of standard bilirubin solution, but the human albumin is expensive and not available commercially in this country. Therefore, authors substituted human albumin with unhemolysed pooled human serum from healthy donors and compared the results with Eberlein´s method.
In the author´s method the maximum absorption spectrum of UE and UB fractions is identical to that of Eberlein´s method but the spectrum of LB is 430㎛. The bilirubin concentration and the optical density show straight line correlation concordant with the Lambert-Beer´s law. The bilirubin value by the author´s method corresponds well with that of Eberlein´s method. The maternal bloods revealed free bilirubin, bilirubin mono- and diglucuronide values of 0.564±0.266, 0.256±0.044 and 0.924±0.184; the cord blcods 1.111±0.110, 0.362±0.049 and 0.832±0.053 mg/100ml respectively by the author´s method.
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